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Recombinant DNA Technology

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This book is designed to meet the needs of B. Sc. Biotech students studying for the very first time in their curriculum. Thus complexity of the matter has been avoided with a view that complete course content has to be completed by the student in limited time period. Every precaution has been taken for accuracy and clarity of the subject matter. We hope that this book will be successful in its objectives and will receive appreciation from students and teachers alike. The subject matter has been presented in a lucid, comprehensive and systematic manner which is easy to understand and also develops writing ability for students to score good marks in upcoming examination.

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SKU: BT301 Categories: ,

Introduction to gene cloning and its uses, tools and techniques: plasmids and other cloning vectors, DNA, RNA, cDNA.

Restriction enzymes and other reagents, techniques, laboratory requirements, safety measures.

Purification of DNA from bacterial and animal cells, manipulation of purified DNA.

DNA library: Genomic DNA and cDNA libraries, their advantages and limitations

DNA Introduction of DNA into living cells and their screening.

Application of cloning in gene analysis (obtaining clone of a specific gene, studying gene location, structure expression).

Expression of foreign genes in prokaryotes and eukaryotes.

Production of proteins from cloned genes: gene cloning in medicine (pharmaceutical agents such as insulin, growth hormone, plasminogen activator, clotting factors, interferon, recombinant gene therapy for genetic diseases).

Nucleic acid oligonucleotides and immunoscreening of libraries and other probes.

Isolation and characterization of genes

Analysis of DNA by southern blotting

Analysis of RNA by northern blotting

Analysis of proteins by western blot techniques, Dot blots and slot blots.

RFLP, AFLP

PCR: Basic principles and its modification, application and uses.

Site-directed mutagenesis

Weight 100 g

2 reviews for Recombinant DNA Technology

  1. 5 out of 5

    Vivaan Garg

    Books are good and easy to understand. As well as process to purchase of books very easy

  2. 5 out of 5

    vivek agarwal

    Perfect Quality Books….!!

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