Introduction to gene cloning and its uses, tools and techniques: plasmids and other cloning vectors, DNA, RNA, cDNA.
Restriction enzymes and other reagents, techniques, laboratory requirements, safety measures.
Purification of DNA from bacterial and animal cells, manipulation of purified DNA.
DNA library: Genomic DNA and cDNA libraries, their advantages and limitations
DNA Introduction of DNA into living cells and their screening.
Application of cloning in gene analysis (obtaining clone of a specific gene, studying gene location, structure expression).
Expression of foreign genes in prokaryotes and eukaryotes.
Production of proteins from cloned genes: gene cloning in medicine (pharmaceutical agents such as insulin, growth hormone, plasminogen activator, clotting factors, interferon, recombinant gene therapy for genetic diseases).
Nucleic acid oligonucleotides and immunoscreening of libraries and other probes.
Isolation and characterization of genes
Analysis of DNA by southern blotting
Analysis of RNA by northern blotting
Analysis of proteins by western blot techniques, Dot blots and slot blots.
PCR: Basic principles and its modification, application and uses.
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