Introduction to in vitro methods: Terms and definitions, use of growth regulators, beginning of in vitro cultures in India, ovary and ovule culture in vitro pollination and fertilization, embryo culture, embryo rescue after wide hybridization and its applications, endosperm culture and production of triploids.
Introduction to the process of embryogenesis and organogenesis and their practical applications: Clonal multiplication of elite species (micropropagation), maxillary bud, shoot-tip and meristem culture, haploid production and their applications, somaclonal variations and applications (treasure your applications), practical applications of tissue and organ culture (summarizing the practical application of all the above mentioned technique) single cell suspension, culture and their applications in selection of variants mutants with and without treatment (of haploid culture preferably).
Introduction to Protoplast isolation: principle and application, testing of viability of isolated protoplasts, various steps in the regeneration of protoplasts. Somatic hybridization-an introduction, various methods for fusing protoplasts, chemical and electrical, use of markers for selection of hybrid cells, practical applications of somatic hybridization (hybrid vs. cybrids).
Use of plant cell, protoplast and tissue culture of genetic manipulation of plant: introduction to A. tumefaciens, tumor formation on plants using A. tumefaciens (monocots vs. dicots), rot formation using A. rhizogenes, practical application of genetic transformation.
Transgenic plant for the production of human therapeutics, edible vaccines, herbicides, insect resistance, production of secondary metabolites, biotransformation, elicitors, immobilized cells.