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This book is designed to meet the needs of B. Sc. Biotech students studying for the very first time in their curriculum. Thus complexity of the matter has been avoided with a view that complete course content has to be completed by the student in limited time period. Every precaution has been taken for accuracy and clarity of the subject matter. We hope that this book will be successful in its objectives and will receive appreciation from students and teachers alike. The subject matter has been presented in a lucid, comprehensive and systematic manner which is easy to understand and also develops writing ability for students to score good marks in upcoming examination.
Molecular basis of life: Structure of DNA (Watson & Crick model, B DNA and Z DNA in detail): DNA replication both prokaryotes and eukaryotic, DNA recombination molecular mechanisms prokaryotic and eukaryotic.
Insertion elements and transposons. (composite, transposons, Tn 3 transposons)
Organisation of genetic material: Split genes; overlapping genes; pseudo genes; cryptic genes.
Genetic code: Properties of genetic code eodon assignment (chain initiation and chain termination codons), wobble hypothesis.
Structure of prokaryotic genes: Prokaryotic transcription; prokaryotic translation prokaryotic gene expression (lac up catabolic repression).
Structure of eukaryotic genes: Eukaryotic transcription; eukaryotic translation
Prokaryotic gene regulation: operon model for regulation of lac genes: positive control of the lac operon: molecular details of lac operon : regulation of trp operon.
Eukaryotic gene expression: levels of control of gene expression: RNA processing. Transport, MRNA translation, MRNA degradation (nucleases, RNA processing. Transport, MRNA translation, MRNA degradation (nucleases, AU rich sequences) and protein degradation controls (ubiquitin molecule).
Molecular mapping of genome: genetic and physical maps, molecular marker for genomic analysis (RFLP, RAPD, VNTR, STRs).
Genome sequencing: methods of genome sequencing (Maxam and Gillbert method, Sanger method).
