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This book is designed to meet the needs of B. Sc. Biotech students studying for the very first time in their curriculum. Thus complexity of the matter has been avoided with a view that complete course content has to be completed by the student in limited time period. Every precaution has been taken for accuracy and clarity of the subject matter. We hope that this book will be successful in its objectives and will receive appreciation from students and teachers alike. The subject matter has been presented in a lucid, comprehensive and systematic manner which is easy to understand and also develops writing ability for students to score good marks in upcoming examination.
Microscopy: Simple microscopy, phase contrast microscopy, florescence and electron microscopy (TEM and SEM)
Instruments, basis principles and usage: pH meter, absorption and emission spectroscopy, principle and law of absorption and radiation, use of densitometry, fluorimetry, colorimetry, spectrophotometry (visible, uv, infrared), manometry, polarography, centrifugation, atomic absorption, IR, NMR, X-ray crystallography.
Chromatography techniques: Paper chromatography, thin layer chromatography, colomn chromatography, gas chromatography, affinity chromatography, gel filtration, chromotography, ion exchange (paper gel etc.)
Electrophoresis: SDS polyacrylamide electrophoresis, immunoelectro-phoriss, isoelectric focussing.
Fermentaton: Different type of fermenters: principle operating, characteristics of fermenters, computer control of fermentation process.
Radioisotope tracer techniques and autoradiography.
